Studies on radioimmunoassay of metanephrine.
نویسندگان
چکیده
منابع مشابه
Radioimmunoassay for free and conjugated urinary metanephrine.
We describe a convenient and specific radioimmunoassay for urinary metanephrine, in which we used an antiserum generated in rabbits by injecting with synephrine conjugated to bovine serum albumin as described by Grota and Brown [Endocrinology 98, 615 (1976)]. The antiserum is specific for metanephrine and epinephrine. Epinephrine cross reaction is minimized to 1% by adding 2 ng of epinephrine t...
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Sensitive and specific radioimmunoassays of metanephrine and normetanephrine were developed by use of 125I-labeled synephrine and specific metanephrine antibody, and 125I-labeled octopamine and specific normetanephrine antibody. Specific antibody for both metanephrine and normetanephrine was raised in rabbits by immunization with bovine serum albumin conjugated with the corresponding hapten, pr...
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A radioimmunoassay involving an 125I ligand has been developed and applied to the measurement of urinary metanephrine. To validate the clinical usefulness of this assay, we compared measurement of metanephrine by radioimmunoassay and of total urinary metanephrines by the Pisano colorimetric method. The radioimmunoassay is specific for metanephrine, whereas the colorimetric method measures both ...
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I t was shown by a radioimmunoassay t h a t p i g p a n c r e a t i c k a l l i k r e i n can be absorbed i n the r a t i n t e s t i n a l t r a c t . The a b s o r p t i o n seems to o c c u r both by way of l y m p h a t i c s and m e s e n t e r i c v e i n r o u t e . G l a n d u l a r k a l l i k r e i n was shown to be present i n the serum of p i g and man i n c o n c e n t r a t i o n ...
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Serum gastrin levels in patients with pernicious anaemia were measured by immunoassay in the fasting state, following gastric perfusion with 0.9% saline, 0.1N hydrochloric acid, and solutions of increasing acidity, and after the intravenous injection or infusion of secretin. The fasting serum gastrin level was measured in 21 patients with pernicious anaemia and found to be elevated at 1,036 +/-...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1980
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.28.2994